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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Immunol. 2013 Mar 11;190(8):4236–4244. doi: 10.4049/jimmunol.1300046

Figure 2. Metalloproteinase blockade by GM6001 delays monocyte transendothelial migration under static and flow conditions.

Figure 2

A. Human monocytes were added to unactivated HUVEC monolayers in the presence of DMSO or GM6001 under static conditions and observed using time-lapse video microscopy every 37.8 s. Individual monocytes migrating on the apical surface of the monolayers were tracked until they complete migration across the monolayers. Monocytes that complete their transendothelial migration up to each time point are expressed as % of total monocytes in the field. In one experiment, an average of 50 monocytes were evaluated for DMSO and 46 for GM6001. B. Monocyte transendothelial migration under flow conditions in the presence of DMSO (Video 1) or GM6001 (Video 2) was analyzed as described in A. Monocytes were drawn across pre-activated HUVEC monolayers (10 ng/ml TNF-α for 4–6 h) at 0.5 dyn/cm2 in flow medium containing DMSO or GM6001. Pretreatment with DMSO or GM6001 was 30 min for HUVECs and 5 min for monocytes. Time-lapse video microscopy was performed every 10 s for 30 min. Means ± SD of 3 different experiments are shown, *p < 0.05, **p <0.01, and ***p <0.005 (paired t test).