Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Clin Exp Metastasis. 2012 Dec 30;30(5):579–594. doi: 10.1007/s10585-012-9562-5

Figure 2.

Figure 2

In vivo passage of ID8 murine ovarian carcinoma cells in C57Bl6 mice result in the spontaneous generation of aggressive cells. (A) Schematic summarizing the intraperitoneal (IP) injection of ID8 cells, re-isolation from ascites after 43 days, growth and expansion in anchorage-independent (poly-HEMA-coated plates) for 4 weeks with the resulting pooled population of cells termed ID8-IP. (B) Adherent proliferation of 50,000 ID8 or ID8-IP cells over 6 days. (C) Anchorage-independent growth of 250,000 ID8 or ID8-IP cells on poly-HEMA coated plates over 6 days. (B and C) Values are means (+/- SD) of triplicate points, (** p<0.01, *** p<0.001). (D) Phase contrast images of ID8 or ID8-IP cells under adherent and confluent (Day 5) or suspended conditions (Day 6). Scale is 70 μM. (E) Soft agar colony number of ID8 or ID8-IP cells after 7 days. Values are means (+/- SD) of triplicate points, (** p<0.01). Scale is 3 mm. (F) Cell lysates were prepared from ID8 and ID8-IP cells growing under adherent or suspended conditions after 3 days. Total protein was normalized for actin and E-cadherin blotting was performed on the same membrane. Phospho-specific immunoblotting for FAK Y397 (pY397) or c-Src Y416 (pY416) phosphorylation was performed and the membranes were re-probed for total FAK or c-Src, respectively.