Table I.
The primary antibodies used for immunohistochemistry.
| Antibody target | Company | Catalogue number | Dilution | Retrieval solution pH* | Positive control |
|---|---|---|---|---|---|
| GLUT-1 | Abcam, Cambridge, UK | Ab652 | 1:200 | Low pH | Kidney cancer |
| GLUT-2 | Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA | Sc-9117 | 1:100 | Low pH | Pancreas |
| GLUT-3 | Abcam, Cambridge, UK | Ab53095 | 1:200 | High pH | Testis |
| GLUT-4 | AbD Serotec, Oxford, UK | 4670-1704 | 1:1000 | High pH | Salivary gland |
| HIF-1α | Novus Biologicals, Cambridge, UK | NB100–499 | 1:300 | High pH | Placenta |
| Caspase-3 | Cell Signaling Technology, Danvers, MA, USA | 9664 | 1:100 | High pH | Large cell lymphoma |
*Low pH: citrate buffer, pH 6.1 (K8005, Dako, Glostrup, Denmark). High pH: Tris/EDTA, pH 9 (K8004, Dako, Glostrup, Denmark).