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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: J Immunol. 2012 May 16;188(12):6184–6193. doi: 10.4049/jimmunol.1102711

Figure 1. B27 homodimers bind to LILRB2 more strongly than B27 heterotrimers.

Figure 1

A. FACS stain of peripheral blood monocytes with HLA-B27 dimer and heterotrimer tetramers and HLA-A3 and HLA-B8 heterotrimer tetramers (left hand panel). Monocyte staining with B27 dimer tetramers preincubated with isotype control MAb, HC10 or anti-LILRB2 MAbs (right hand panel). Representative stains from one of three independent experiments. EX PE: extravidin PE. B SPR analysis of LILRB2 binding to B27 heterotrimer. Non-linear fit of the Langmuir binding isotherm for LILRB2 binding to the B27 heterotrimer, inset is a Scatchard plot for LILRB2 binding to B27 heterotrimer. Non-linear Langmuir fit of LILRB2 binding to B27 heterotrimer and scatchard analysis yields a KD of 21μM. Representative data from three independent experiments. C. SPR analysis of LILRB2 binding to B27 homodimers. Non-linear Langmuir fit of LILRB2 binding to B27 homodimer and scatchard analysis (inset) yields a KD of 2.5μM. Representative data from three independent experiments.