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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: J Immunol. 2012 May 16;188(12):6184–6193. doi: 10.4049/jimmunol.1102711

Figure 6. B27-expressing 221 cells inhibit production of IL-2 by LILRB2-transduced Jurkat T cells to a greater extent than 221 cells expressing control HLA-class 1.

Figure 6

A. FACS staining of LILRB2-transduced Jurkat T cells with anti-LILRB2 MAb A and B27 dimer, HLA-A3 and HLA-B8 tetramers B. C IL2 secretion by LILRB2-transduced Jurkat T cells stimulated with staphylococcal enterotoxin E (SEE) presented by parental LBL721.221 (221) cells (1112pg/ml±77) and 221 cells transfected with HLA-B27 (202pg/ml±59), the C67S mutant of HLA-B27 (1125pg/ml±74), HLA-B35 (847pg/ml±32) or HLA-A3 (1017pg/ml±103). IL2 secretion by parental non-transduced Jurkat T cells was 1095pg/ml±120, 1072pg/ml±306, 1112pg/ml±63 and 941pg/ml;±139 for T cells stimulated with SEE presented by 221, 221B27, 221B27C67S and 221B35 cells respectively (results not shown). Results are representative data from one experiment of three independent experiments.