Table 1.

Results of TRAP Assay and Cloning

Note.—Telomere type was determined from the nucleotide periodicity of the TRAP products (lower case letters) or from cloned TRAP products (upper case letters). Primers: a, pSSyF; b, 47F; c, GG(21); d, TS21; e, CAMV; f, TELPR30-3A; g, TELPR; h, HUTC; i, T4AG2-C; j, T4AG2-PR; k, CHTRTTRAPRev1; l, TTATAG3-C; n.a., not analyzed. Minisatellite variants refer to number of indicated telomere motifs detected among telomerase products, the major telomere types are indicated in bold. Errors classified as T- or G-slippage are additional T or G nucleotides incorporated into the reiterated unit. Other errors, mostly nucleotide deletion or A/G substitution, are considered nucleotide misincorporations (mismatches).