Table 3.
Observed and predicted values of endoglucanase activity for the independent variables pH and temperature used in CCRD, from the crude enzyme preparation of Streptomyces misionensis PESB-25.
| Run | pH/Coded level | Temperature (°C)/Coded level | Endoglucanase activity (U·mL−1) Observed |
Endoglucanase activity (U·mL−1) Predicted |
|---|---|---|---|---|
| 1 | 3.6 (−1) | 44 (−1) | 1.02 | 0.96 |
| 2 | 6.4 (+1) | 44 (−1) | 0.77 | 0.78 |
| 3 | 3.6 (−1) | 66 (+1) | 1.53 | 1.42 |
| 4 | 6.4 (+1) | 66 (+1) | 0.81 | 0.77 |
| 5 | 3.0 (−1.41) | 55 (0) | 1.21 | 1.31 |
| 6 | 7.0 (1.41) | 55 (0) | 0.72 | 0.72 |
| 7 | 5.0 (0) | 40 (−1.41) | 0.78 | 0.79 |
| 8 | 5.0 (0) | 70 (1.41) | 1.02 | 1.11 |
| 9 | 5.0 (0) | 55 (0) | 1.27 | 1.23 |
| 10 | 5.0 (0) | 55 (0) | 1.26 | 1.23 |
| 11 | 5.0 (0) | 55 (0) | 1.18 | 1.23 |
| 12 | 5.0 (0) | 55 (0) | 1.20 | 1.23 |