Table 5.
Effect of metal ions on endoglucanase activity. Enzyme was produced by S. misionensis PESB-25 grown on 1.0% (w/v) SCB and 1.2% (w/v) CSL.
| Iona | Relative activity (%)* |
Endoglucanase activity (U·mL−1) |
|---|---|---|
| Control (no addition) | 100.0 | 1.72 |
| NaCl | 133.2 ± 2.0 | 2.23 |
| CuSO4 | 140.6 ± 0.3 | 2.30 |
| MgSO4 | 126.6 ± 0.9 | 2.18 |
| CoSO4 | 161.2 ± 0.6 | 2.73 |
| MnSO4 | 201.5 ± 0.1 | 3.48 |
| FeSO4 | 131.1 ± 0.1 | 2.34 |
| CaCl2 | 137.6 ± 2.3 | 2.25 |
| KCl | 125.3 ± 4.9 | 2.17 |
| BaCl2 | 109.3 ± 0.9 | 1.97 |
aThe final concentration in the reaction mixture was 2 mM.
*Values are based on Mean ± SD of 3 individual observations.