Fig. 2.

Butein inhibits adipogenesis in 3T3-L1 cells and MEFs. A: Commercially available butein suppressed lipid accumulation in 3T3-L1 cells in a dose-dependent manner. B: 3T3-L1 cells were differentiated and treated with butein for 7 days, and protein expression of PPARγ, aP2, and β-actin was measured by Western blot analysis. C: 3T3-L1 cells were differentiated and treated with various doses of butein, and mRNA expression of PPARγ, LPL, aP2, and C/EBPα was measured by real-time PCR. D, E: Butein inhibited adipogenesis of freshly isolated MEFs and primary bone marrow cells. MEFs (D) or bone marrow cells (E) were differentiated and treated with 10 μM of butein for 7 days and mRNA expression of PPARγ and C/EBPα was measured by real-time PCR. Data shown represent the mean ± SEM from three independent experiments. Statistical significance was determined relative to a control by the Student's t-test (*P < 0.05; **P < 0.005; ***P < 0.0005). Ctrl, control; Norm., normalized; But, butein.