Fig. 3.

Butein is a potent novel anti-adipogenic compound. A: C3H10T1/2 cells were differentiated in the presence of various concentrations (1, 5, 10, 20, and 40 μM) of butein, genistein, and resveratrol for 7 days. B: Differentiated cells were stained with Oil Red O, and lipid accumulation was quantified by measuring the extracted dye at 520 nm. C: 3T3-L1 cells were differentiated with DMI and various doses of butein, resveratrol, and genistein for 7 days, and lipid accumulation was quantified. Data shown represent the mean ± SEM from three or more independent experiments. Statistical significance was determined relative to a control by the Student's t-test (*P < 0.05; **P < 0.005; ***P < 0.0005). Ctrl, control; DMI, dexamethasone, IBMX, insulin.