TABLE 2.

Effect of mutation of Lys⁴⁷⁶ on CYP27A1 binding of cholesterol and C3S in the absence and presence of PLs

	Kd (μM)				ΔAmax/μmol P450
	Cholesterol		C3S		Cholesterol		C3S
CYP27A1	−DLPC	+DLPC	−DLPC	+DLPC	−DLPC	+DLPC	−DLPC	+DLPC
WT	0.6 ± 0.2	0.3 ± 0.1	0.4 ± 0.2	0.8 ± 0.1	62 ± 2	40 ± 1	225 ± 2	275 ± 8
K476L	LSR	LSR	1.1 ± 0.1	5.5 ± 0.4	<10	<10	217 ± 7	312 ± 1
K476R	1.1 ± 0.1	1.2 ± 0.1	1.4 ± 0.1	3.5 ± 0.1	85 ± 0.8	42 ± 1	345 ± 6	342 ± 1

LSR, low spectral response. Values are the mean ± SD of triplicate measurements.