Fig. 3.

Rb1 treatment regulates gene expression in cultured hepatic cells (A, B) and rat liver tissues (C). In in vitro study, hepatocytes were cultured in HepatoZYME-SFM for 18 h, and the expression of genes encoding enzymes and proteins that function in lipogenesis or lipolysis was determined by qPCR following an additional 6 h incubation with vehicle (PBS), Rb1 (10 μM), compound C (20 μM), or compound C (20 μM) plus Rb1 (10 μM) for an additional 6 h. The compound C was added into the medium 30 min prior to Rb1 treatment. The genes encoding enzymes/proteins that function in lipogenesis included SREBP1c, FAS, ACC, and SCD-1; and the genes encoding enzymes/proteins that function in lipolysis included PGC-1α, PPAR, CPT1A, and Acox1. Data are means ± S.E. (n = 3–5). *P < 0.05 and **P < 0.01, Rb1-treated cells versus PBS-treated cells. ^#P < 0.05 and ^##P < 0.01, compared with all other groups.