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. 2013 May;54(5):1439–1447. doi: 10.1194/jlr.M036186

TABLE 2.

Optimized reaction conditions for the production of monohydro(pero)xy and dihydro(pero)xy products

Monohydro(pero)xy Dihydro(pero)xy
Enzyme amount 2 mg 5 mg
Substrate concentration 0.10 mM 0.10 mM
Temperature 20°C 20°C
pH pH 12 pH 9
Buffer concentration 50 mM 50 mM
Oxygenation No oxygenation No oxygenation
Time 15 min 15 min