TABLE 2.
Optimized reaction conditions for the production of monohydro(pero)xy and dihydro(pero)xy products
Monohydro(pero)xy | Dihydro(pero)xy | |
Enzyme amount | 2 mg | 5 mg |
Substrate concentration | 0.10 mM | 0.10 mM |
Temperature | 20°C | 20°C |
pH | pH 12 | pH 9 |
Buffer concentration | 50 mM | 50 mM |
Oxygenation | No oxygenation | No oxygenation |
Time | 15 min | 15 min |