TABLE 4.
Product characterization
| TOF-MS |
|||||
| Substrate | Product | GC-MS,a Major Ions | Expected (m/z) | Actual (m/z) | UV-vis (nm) |
| ARA | 15-HETE | 73, 117, 173, 401, 457 | 357.24002c | 357.22519 | 236.6 |
| 5,15-diHETE | 73, 173, 261, 399, 489, 545 | 373.23493c | 373.22115 | 243.4 (shoulder at 226.9) | |
| 8,15-diHETE | 73, 173, 303, 359, 489, 545 | 373.23493c | 373.23395 | 269.0 (suppressed maxima at 259.6 and 279.5) | |
| EPA | 15-HEPA | 73, 117, 173, 401, 457 | 317.21222b | 317.57493 | 235.4 |
| 5,15-diHEPA | 73, 173, 261, 399, 489, 545 | 371.21928c | 371.20427 | 244.3 (shoulder at 225.8) | |
| 8,15-diHEPA | 73, 173, 303, 359, 489, 545 | 371.21928c | 371.20417 | 269.5 (suppressed maxima at 260.4 and 280.3) | |
| DPAn-6 | 17-HDPAn-6 | 73, 117, 173, 429, 485 | 345.24297b | 345.37750 | 232.8 |
| 10,17-diHDPAn-6 | 73, 173, 331, 359, 427, 517, 573 | 399.25113c | 399.48680 | 269.0 (suppressed maxima at 259.8 and 280.0) | |
| DPAn-3 | 17-HDPAn-3 | 73, 117, 173, 429, 485 | 345.24297b | 345.37657 | 236.5 |
| 7,17-diHDPAn-3 | 73, 117, 173, 289, 402, 517, 573 | 399.25058c | 399.24808 | 243.6 (shoulder at 227.6) | |
| DHA | 17-HDHA | 73, 117, 173, 429, 485 | 343.22787b | 343.44719 | 236.8 |
| 7,17-diHDHA | 73, 117, 173, 289, 402, 517, 573 | 397.23493c | 397.21042 | 243.8 (shoulder at 227.6) | |
| 10,17-diHDHA | 73, 117, 173, 331, 359, 427, 517, 573 | 397.23493c | 397.23907 | 269.8 (suppressed maxima at 260.4 and 280.5) | |
a
All products analyzed as hydrogenated, trimethylsilyl derivatives.
b
Free acid [M-H]− ion in negative ion mode.
c
Methyl ester [M+Na]+ ion in positive ion mode.