rAAV-CYP2J2 delivery led to aortic CYP2J2 overexpression and increased EET synthesis in vivo. A: CYP2J2 was overexpressed in aortic tissues after rAAV-CYP2J2 injection. rAAV-CYP2J2 injection increased the serum concentrations of 11,12-EET and corresponding 11,12-DHET (B), as well as 14,15-EET and 14,15-DHET (C). In addition, Ang II infusion led to a marked reduction of serum 11,12- and 14,15-EETs, and 11,12- and 14,15-DHETs (B, C). rAAV-CYP2J2 delivery also markedly elevated the urine concentrations of 11,12-EET and 11,12-DHET (D), as well as 14,15-EET and 14,15-DHET (E) (n = 8 for each group; **P < 0.01 vs. control; ††P < 0.01 vs. Ang II + rAAV-GFP).