Fig. 9.

Comparison of the intermediates involved in anaerobic testosterone catabolic pathway (demonstrated in S. denitrificans DSMZ 18526) with those of anaerobic cyclohexanol catabolism (by Alicycliphilus denitrificans DSMZ 14773). The scheme of anaerobic cyclohexanol catabolic pathway is based on Jin et al. (36). 2-cyclohexenone hydratase belongs to the xanthine oxidase family, which is inactivated by KCN. This bifunctional molybdoenzyme (MhyADH) also catalyzes the subsequent alcohol oxidation to form 1,3-cyclohexanedione. According to our data, KCN effectively inhibited the hydration reaction of 1-testosterone.