TABLE 3.
13C-NMR chemical shifts (δC, ppm) of ring cleavage intermediates involved in aerobic or anaerobic testosterone catabolic pathways in S. denitrificans cells
13C | 3,17-DHSA | 2,3-SAOA |
1 | 131.4 | 216.8 |
2 | 113.0 | 25.5 |
3 | 154.1 | 176.6 |
4 | 116.1 | 40.9 |
5 | 142.7 | 42.5 |
6 | 31.5 | 28.0 |
7 | 27.6 | 30.4 |
8 | 50.9 | 36.1 |
9 | 212.7 | 50.5 |
10 | 128.4 | 57.1 |
11 | 38.4 | 24.2a |
12 | 35.9 | 37.6 |
13 | 43.7 | 44.1 |
14 | 50.1 | 52.0 |
15 | 24.5 | 24.2a |
16 | 31.5 | 32.1 |
17 | 80.8 | 82.4 |
18 | 11.1 | 11.6 |
19 | 18.7 | 9.9 |
The signals of C-11 and C-15 of 2,3-SAOA are undistinguishable.