Table 1.
Heart viability assessment
| Solution | 0 min | 10 min | 20 min | 30 min | 40 min | 50 min | 60 min | 70 min | 80 min |
|---|---|---|---|---|---|---|---|---|---|
| UW | 100±0 | 80.05±4.33c,d | 58.19±6.18c,d | 38.62±1.82c,d | 32.37±1.21c,d | 25.67±1.26c,d,e,f | 24.75±1.60c,d,e,f | 23.08±1.16c,d,e,f | 21.28±1.31c,d,e,f |
| ETK | 100±0 | 77.49±8.19c,d | 54.41±7.61b,c | 37.45±4.45c.d | 29.83±3.51c,d | 24.52±2.98c,d,e,f | 21.77±2.78c,d,e,f | 20.65±3.23c,d,e,f | 19.96±2.51c,d,e,f |
| HTK | 100±0 | 66.44±5.95 | 47.20±10.01a | 29.04±4.54a | 21.45±5.80 | 13.18±3.09 | 8.70±1.66 | 5.61±1.26b,c,d | 5.61±0.63c,d,e,f |
| EC | 100±0 | 74.50±3.35b,c | 48.07±1.94a | 39.44±2.44c,d | 28.44±2.18b,c | 23.88±1.82c,d,e,f | 20.87±1.10c,d,e,f | 15.65±1.24e | 15.64±1.09e |
| LR | 100±0 | 39.45±7.08 | 19.42±2.90 | 11.35±1.95 | 9.36±2.05 | 7.73±1.74 | 6.77±1.54 | 5.18±0.97 | 4.97±1.29 |
| Saline | 100±0 | 42.56±6.31 | 21.59±3.15 | 17.41±3.22 | 11.56±2.16 | 9.81±2.19 | 8.09±1.61 | 6.57±1.28 | 5.72±1.19 |
Bioluminescent imaging using the IVIS system assessed heart viability. All samples were exposed to organ preservation solutions and saline (control) at 4°C. Data are representative of seven independent experiments.
p Values estimated by repeated measure one-way analysis of variance (ANOVA) and one-way ANOVA were a: <0.05 for LR, b: <0.05 for saline, c: <0.01 for LR, d: <0.01 for saline, e: <0.05 for HTK and f: <0.01 for HTK.
EC, Euro-Collins; ETK, extracellular-trehalose-Kyoto solution; HTK, histidin-tryptophan-ketoglutarat solution; IVIS, in vivo imaging system; LR, lactated Ringer's; UW, University of Wisconsin.