TABLE 5.
TOP 20 CpG SITES WITH DIFFERENTIAL METHYLATION BY CURRENT SYSTEMIC STEROID USE IN SUBJECTS WITH OBSTRUCTIVE LUNG DISEASE (MODEL 1)
| ICGN |
EOCOPD |
||||||
| CpG | Gene Symbol | Difference in Mean Methylation* | P Value† | Difference in Mean Methylation* | P Value‡ | Liptak Combined P Value | Overlaps with Model 1 (full cohort) |
| cg25600606 | HIPK3 | −0.15 | 5.93 × 10−27 | −0.07 | 2.90 × 10−3 | 4.47 × 10−28 | Yes |
| cg19906550 | SLC22A18 | −0.10 | 3.15 × 10−26 | −0.05 | 1.79 × 10−3 | 1.24 × 10−27 | Yes |
| cg27461196 | FXYD1 | −0.15 | 3.45 × 10−26 | −0.07 | 1.92 × 10−3 | 1.48 × 10−27 | Yes |
| cg08700306 | LRP3 | −0.09 | 1.16 × 10−25 | −0.05 | 1.60 × 10−3 | 3.86 × 10−27 | Yes |
| cg18884741 | RABGEF1 | −0.16 | 1.90 × 10−26 | −0.06 | 2.46 × 10−2 | 2.99 × 10−26 | Yes |
| cg17709873 | LTA | 0.10 | 3.03 × 10−25 | 0.06 | 4.43 × 10−3 | 3.41 × 10−26 | Yes |
| cg09868035 | ABHD16B | −0.09 | 3.19 × 10−25 | −0.04 | 6.52 × 10−3 | 5.96 × 10−26 | Yes |
| cg01526089 | P2RX1 | −0.13 | 2.71 × 10−25 | −0.05 | 8.29 × 10−3 | 7.08 × 10−26 | Yes |
| cg07730301 | ALDH3B1 | −0.09 | 5.47 × 10−25 | −0.04 | 5.41 × 10−3 | 7.82 × 10−26 | Yes |
| cg26215727 | SCNN1A | −0.09 | 4.52 × 10−23 | −0.05 | 1.09 × 10−4 | 8.78 × 10−26 | Yes |
| cg00795812 | PDCD1 | 0.09 | 2.11 × 10−25 | 0.04 | 1.30 × 10−2 | 1.06 × 10−25 | Yes |
| cg17823175 | AZU1 | −0.12 | 2.63 × 10−23 | −0.06 | 1.28 × 10−3 | 6.02 × 10−25 | Yes |
| cg25634666 | FOLR3 | −0.10 | 1.14 × 10−23 | −0.05 | 4.40 × 10−3 | 1.13 × 10−24 | Yes |
| cg06270401 | DYRK4 | −0.12 | 5.48 × 10−24 | −0.05 | 1.05 × 10−2 | 1.75 × 10−24 | Yes |
| cg00974864 | FCGR3B | −0.11 | 3.60 × 10−23 | −0.05 | 2.87 × 10−3 | 2.03 × 10−24 | Yes |
| cg21019522 | SLC22A18 | −0.08 | 9.62 × 10−23 | −0.04 | 3.12 × 10−3 | 5.84 × 10−24 | Yes |
| cg08368934 | GPR97 | −0.10 | 2.30 × 10−23 | −0.04 | 1.10 × 10−2 | 7.35 × 10−24 | Yes |
| cg24211388 | AIF1 | −0.12 | 2.48 × 10−23 | −0.05 | 1.14 × 10−2 | 8.30 × 10−24 | Yes |
| cg10758292 | DEFA1 | −0.13 | 2.66 × 10−22 | −0.06 | 2.85 × 10−3 | 1.41 × 10−23 | Yes |
| cg05681757 | FGD4 | −0.09 | 6.46 × 10−21 | −0.05 | 2.30 × 10−4 | 2.51 × 10−23 | Yes |
Definition of abbreviations: EOCOPD = Early Onset COPD Study; ICGN = International COPD Genetics Network.
*
Difference in mean methylation = (mean methylation subjects on steroids) − (mean methylation subjects not on steroids). Methylation values range from 0 to 1.
†
P value from regression Model 1 (adjusted for age, sex, and FEV1 % predicted).
‡
One-sided P value from regression model.