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Sensitivity determination of the developed real-time PCR assay. (A) Amplification in samples containing successive 10-fold dilutions of CoHV-1 standard isolate. Concentration of DNA in each sample was given. (B) Amplification in samples containing serial dilutions (108 – 1E8, 106 – 1E6, 104– 1E4, 102 – 1E2 and 101 – 1E1) copies of pGW1126 plasmid. ΔRn is an increment of fluorescent signal during successive cycles.
