Table 2.
CD4+CD8+subtype of T cells from immunization mice were measured using flow cytometry
| Immunization regimena | CD4+(%)b | CD8+(%)b | CD4+/CD8+ |
|---|---|---|---|
| PBS |
26.71±0.22 |
18.67±0.92 |
1.43±0.23 |
| pcDNA3.1 |
27.62±0.25 |
24.71±0.85 |
1.12±0.29 |
| pSAG1 |
26.45±0.34 |
26.37±0.98 |
1.00±0.35 |
| pSAG1/SAG3 |
24.68±0.14 |
35.01±0.88 |
0.70±0.16 |
| pSAG1/SAG3-CTXA2/B | 25.15±0.23 | 38.55±0.94 | 0.65±0.24 |
a Mice were immunized by i.m. route on day 0 and day 14 and day 28 with PBS, pcDNA3.1, pSAG1, pSAG1/SAG3, pSAG1/SAG3-CTXA2/B.
b The splenocytes culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were stained with FITC-labeled anti-mouse CD8+ monoclonal antibody and PE-labeled anti-mouse CD4+ monoclonal antibody, T lymphocyte subsets were measured using flow cytometry.