Figure 5. Effect of miR-34a inhibitor and miR-34a mimic on cell migration and invasion in HepG2 cells.

HepG2 cells (5×10⁴ cells per well using 6-well-plate) were cultured to 50% confluent and transfected with the miR-34a inhibitor, miR-34a mimic and the controls. The cells were incubated in serum-free medium for another one day 96 h post transfection. Then the cells were trypsinized and added (5×10⁴ cells) to the chamber (6.5 mm in diameter, 8 µm pore size) for both migration and invasion assays. migrating and invasive cells were fixed, stained, counted and the ratio of cell migration and cell invasion was calculated.