Figure 1. Bacterial growth of wild type mc²155 strain and M. smegmatis AS strain.

The cultures were grown in LB broth containing 0.05% Tween 80 at 37°C with different concentration of tetracycline. The OD600 and CFU of the cultures were monitored at interval of 12 h. A. Growth curve of the wild type mc²155 strain; B. Growth curve of the M. smegmatis AS strain; C. CFU of the wild type mc²155 strain; D. CFU of the M. smegmatis AS strain. (▴) The cultures without tetracycline; (▪) The cultures induced with 10 ng/ml tetracycline; (○) The cultures induced with 20 ng/ml tetracycline; (•) The cultures induced with 30 ng/ml tetracycline; (□) The cultures induced with 50 ng/ml tetracycline. The values plotted are the mean value and standard deviation from triplicate experiments.