Figure 2. Expression of GlmM protein in wild type mc²155 strain and M. smegmatis AS strain.

The expressions of M. smegmatis GlmM proteins in wild type mc²155 strain (A) and M. smegmatis AS strain (B) were detected by using anti-GlmM polyclonal antibody. The results showed that there were no obvious differences of GlmM protein expressions in wild type mc²155 strain with or without 20 ng/ml tetracycline. However, compared with uninduced M. smegmatis AS strain, the expression of GlmM protein was 70% decreased in M. smegmatis AS strain when induced with 20 ng/ml tetracycline. M. PageRuler prestained protein ladder; lane 1. GlmM proteins in uninduced cultures; lane 2. GlmM proteins in cultures induced with 20 ng/ml tetracycline.