Table 1. Bacterial strains and plasmids used in this study.
| Strains/Plasmids | Description | Source |
| Strains | ||
| E. coli NovaBlue | Used for cloning and propagation of plasmids | Novagen |
| M. smegmatis mc2155 | Used as a DNA template to amplify DNA fragment of M. smegmatis glmM (420 bp) | ATCC |
| M. smegmatis AS | M. smegmatis mc2155 carrying pMind-glmM-420-AS | This study |
| Plasmids | ||
| pMD18-T | Carries ampR gene; used for cloning PCR product with A at 3′ ends | Takara |
| pMind | Carries kanR gene; used for expression of glmM antisense mRNA | [13] |
| pMD-glmM-420 | The DNA fragment (420 bp) of M. smegmatis glmM was cloned to the EcoRV site of pMD18-T | This work |
| pMind-glmM-420-AS | The DNA fragment (420 bp) of M. smegmatis glmM in pMD-glmM-420 was cloned to the SpeI and NdeI sites of pMind | This work |