Skip to main content
. 2013 Apr 10;8(4):e60937. doi: 10.1371/journal.pone.0060937

Figure 4. RT-PCR of Pax2, Wnt4 and WT1. miRNA let-7e silencing (DIF-Let-7e) and BIO (DIF+BIO) provokes a downregulation of the genes Pax2, Wnt4 and WT1.

Figure 4

Inhibition of PKCβ (DIF-Let-7e+PKCβi) provokes a recovery in the expression of these genes. Levels were normalized to GAPDH and data represent the mean from three independent experiments ±S.D (*) P<0.05 versus DIF, (+) P<0.05 versus DIF-Let7e. Groups: differentiated EBs+control antimiR (DIF), Non treated EBs (CONTROL), differentiated EBs+LNA-antiLet-7e (DIF-Let-7e), differentiated EBs+LNA-antiLet-7e and PKCβ inhibitor (Gö 6983) (DIF-Let-7e+PKCβi), differentiated EBs+BIO (DIF+BIO).