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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: Physiol Genomics. 2011 Aug 30;43(21):1226–1240. doi: 10.1152/physiolgenomics.00034.2011

Table 2.

Evaluation of quality filter criteria in microarrays

Sample Scale factor % P Ratio 3′/5′ actin Ratio 3′/5′ GAPDH
(validation threshold) Max/Min<3 >20 and Max-Min<15 <4 <4
D2-C 0.504 60.4 1.25 0.77
D4-DT 0.582 59.5 1.30 0.93
D34-C 0.661 59.3 1.26 0.74
D40-DT 0.643 60.7 1.25 0.87
D48-C 0.635 60.6 1.25 0.78
D50-DT 0.486 60.8 1.25 0.81
D52-C 0.544 60.2 1.25 0.76
D58-DT 0.606 60.0 1.27 0.77
Mean ± se 0.580 ± 0.023 60.19 ± 0.20 1.26 ± 0.006 0.804 ± 0.004

Each sample and hybridization experiment underwent a quality control evaluation, including percentage of probe sets reliably detecting between 59.3% and 60.8% Present call, and 3′-5′ ratios of actin and GAPDH genes <1.3 and <1, respectively. %P: percentage of genes detected as present by Microarray Suite 5.1 software; C: control pup; DT: double-transgenic pup.