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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: Physiol Genomics. 2011 Aug 30;43(21):1226–1240. doi: 10.1152/physiolgenomics.00034.2011

Table 3.

Morphometric analysis of the lungs of doxycycline-treated pups

P4 P8 P16
n controls 10 double transgenic 9 controls 8 double transgenic 8 controls 14 double transgenic 8
VL (cm3) 0.135 ± 0.009 0.152 ± 0.010 0.249 ± 0.014 0.271 ± 0.017 0.376 ± 0.015 0.392 ± 0.027
Sva (cm2/cm3) 294 ± 12 277 ± 10 340 ± 18 293 ± 17 361 ± 12 358 ± 17
Vvp (%) 0.964 ± 0.007 0.986 ± 0.019 0.989 ± 0.002 0.992 ± 0.002 0.995 ± 0.001 0.992 ± 0.002
MLI (μm) 13.8 ± 0.5 14.6 ± 0.5 12.0 ± 0.7 14.0 ± 0.8 11.3 ± 0.4 11.3 ± 0.5
Sa (cm2) 38 ± 3 41 ± 2 83 ± 6 79 ± 7 135 ± 7 139 ± 10

Treatment with doxycycline was the same as that for animals used for microarray analysis. Pups were collected from 3 or 4 litters per stage at postnatal days 4, 8 or 16. Data are mean ± SE. Abbreviations: n, number of individuals per group; VL, lung volume; Sva, alveolar surface density; Vvp, volumetric density of lung alveolar parenchyma; MLI, mean linear intercept; Sa, absolute surface area of airspaces. No significant difference was observed between the 2 groups for any parameter either at P4, P8 or P16 (two-tailed t-test).