Table 5.
Results of array analysis: genes down regulated at least 25% by FGF18 induction in the 4 double-transgenic mouse pups as compared to their respective littermate single-transgenic controls (one-tailed paired t test).
| Accession # | Name | Funct. Cat. | log2-fold change | fold change | P value |
|---|---|---|---|---|---|
| NM_030888 | C1q and tumor necrosis factor related protein 3 (C1qtnf3/cartonectin) | Signal, Lipog | −1.59897 | x 0.33 | 0.0094 |
| NM_030601 | Ca(2+)-sensitive chloride channel 2 (Cacc) | Transport, Epm | −1.39070 | x 0.381 | 0.0039 |
| NM_009127 | stearoyl-Coenzyme A desaturase 1 (Scd1) | Enz, Lipog | −1.28176 | x 0.411 | 0.0088 |
| AY083616 | ATP-binding cassette transporter ABCA3 | Transport, Epm | −1.05604 | x 0.481 | 0.0004 |
| NM_008760 | osteoglycin (Ogn) | ECM | −1.04551 | x 0.484 | 0.0088 |
| NM_007986 | fibroblast activation protein (Fap/seprase) | Enz, ECM, Migr | −1,02764 | x 0.491 | 0.0002 |
| AK007703 | homolog to ATP-binding cassette transporter, sub-family A, member 3 | Transport, Epm | −0.97681 | x 0.508 | 0.0003 |
| NM_010025 | doublecortin | Migr | −0.94387 | x 0.52 | 0.0103 |
| NM_021400 | proteoglycan 4 (Prg4) | ECM | −0,92209 | x 0.528 | 0.0187 |
| NM_009181 | sialyltransferase 8 (alpha-2, 8-sialytransferase) B (Siat8b) | Enz, Glycoconj | −0.89706 | x 0.537 | 0.0265 |
| NM_009608 | actin, alpha, cardiac (Actc1) | MCm | −0.88653 | x 0.541 | 0.0397 |
| NM_009393 | troponin C, cardiac/slow skeletal (Tncc1) | MCm | −0.86216 | x 0.55 | 0.0262 |
| NM_013496 | cellular retinoic acid binding protein I (Crabp1) | Retino metab | −0.85952 | x 0.551 | 0.0373 |
| NM_011674 | UDP-glucuronosyltransferase 8 | Enz, Detox | −0.82905 | x 0.563 | 0.0247 |
| NM_013808 | cysteine-rich protein 3 (Csrp3) | MCm | −0.80843 | x 0.571 | 0,0064 |
| NM_010867 | myomesin 1 (Myom1) | MCm | −0.79768 | x 0.575 | 0.0049 |
| AF311699 | c-type lectin protein MT75 (chondrolectin) | ECM | −0.79070 | x 0.578 | 0.0003 |
| NM_015825 | SH3-binding domain glutamic acid-rich protein (Sh3bgr) | MCm | −0.78637 | x 0.581 | 0.0121 |
| NM_033268 | actinin alpha 2 (Actn2) | MCm | −0.77289 | x 0.585 | 0.0405 |
| NM_013834 | secreted frizzled-related sequence protein 1 (Sfrp1) | Prolif, EMT | −0.76298 | x 0.589 | 0.0005 |
| NM_010858 | myosin light chain, alkali, cardiac atria (Myla) | MCm | −0.75605 | x 0.592 | 0.0373 |
| NM_018870 | phosphoglycerate mutase 2 (Pgam2) | Enz, Inter metab | −0.74443 | x 0.597 | 0.0268 |
| NM_011620 | troponin T3, skeletal, fast (Tnnt3) | MCm | −0.73578 | x 0.6 | 0.0170 |
| NM_011443 | high mobility group box protein (SRY-box containing gene 2, Sox2) | Trancrip, Epm | −0.72852 | x 0.6 | 0.0028 |
| NM_013467 | aldehyde dehydrogenase family 1, subfamily A1 (Aldh1a1) | Retino metab | −0.71960 | x 0.607 | 0.0216 |
| NM_011111 | plasminogen activator inhibitor, type II (Serpinb2) | Signal, Migr | −0.71960 | x 0.607 | 0.0019 |
| NM_016689 | aquaporin-3 | Transport, Epm | −0.69277 | x 0.619 | 0.0330 |
| NM_009944 | cytochrome c oxidase subunit VIIa-H precursor (Cox7ah) | Enz, Detox | −0.68876 | x 0.62 | 0.0211 |
| NM_011395 | solute carrier family 22 (organic cation transporter), member 3 (Slc22a3) | Transp | −0.68500 | x 0.622 | 0.0149 |
| NM_010856 | Mouse alpha cardiac myosin heavy chain (Myhca) | MCm | −0.67333 | x 0.627 | 0.0422 |
| NM_009922 | calponin 1 (Cnn1) | MCm | −0.66432 | x 0.631 | 0.0093 |
| BB193413 | aquaporin 4 (Aqp4) | Transport, Epm | −0.65006 | x 0.637 | 0.0335 |
| AF274959 | uteroglobin related protein 1A/secretoglobin (Scgb3a2) | Transport, Epm | −0.64771 | x 0.638 | 0.0196 |
| NM_008973 | pleiotrophin | Sign | −0.64583 | x 0.639 | 0.0007 |
| AF128849 | cytochrome P450 2B10 related protein (Cyp2b20) | Enz, Detox | −0.63401 | x 0.644 | 0.0318 |
| NM_010356 | glutathione S-transferase, alpha 3 (Gsta3) | Enz, Detox | −0.63049 | x 0.646 | 0.0207 |
| NM_033597 | myeloblastosis oncogene (Myb) | Transcript | −0.61924 | x 0.65 | 0.0174 |
| NM_021396 | butyrophilin-like protein (Btdc) | Immun | −0.61661 | x 0.652 | 0.0013 |
| NM_008182 | glutathione S-transferase, alpha 2 (Yc2) (Gsta2) | Enz, Detox | −0.61598 | x 0.652 | 0.0069 |
| NM_009405 | troponin I, skeletal, fast 2 (Tnni2) | MCm | −0.61569 | x 0.653 | 0.0417 |
| NM_011704 | vanin 1 (Vnn1) | Detox | −0.60899 | x 0.656 | 0,0001 |
| NM_009676 | aldehyde oxidase 1 (Aox1) | Enz, | −0.60447 | x 0.658 | 0.0047 |
| NM_023785 | pro-platelet basic protein (Ppbp) | Signal, Immun | −0.59965 | x 0.66 | 0.0094 |
| NM_018778 | claudin 8 | Epm | −0.59480 | x 0.662 | 0.0003 |
| NM_009994 | cytochrome P450, 1b1, benz(a)anthracene inducible (Cyp1b1) | Enz, Detox | −0.59413 | x 0.662 | 0.0115 |
| NM_008165 | glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1) | Recept | −0.59078 | x 0.664 | 0.0020 |
| NM_008592 | forkhead box C1 (Foxc1) | Trancript | −0.59037 | x 0.664 | 0.0382 |
| NM_007710 | creatine kinase, muscle (Ckmm) | Enz, MCm | −0.58630 | x 0.666 | 0.0162 |
| NM_007617 | caveolin 3 | Signal, MCm | −0.58227 | x 0.668 | 0.0273 |
| NM_007933 | enolase 3, beta muscle (Eno3) | Enz, MCm | −0.57902 | x 0.669 | 0.0328 |
| NM_009998 | cytochrome P450, 2b10, phenobarbitol inducible, type b (Cyp2b10) | Enz, Detox | −0.57377 | x 0.672 | 0.0312 |
| NM_008469 | keratin complex 1, acidic, gene 15 (Krt1-15) | Epm | −0.56978 | x 0.674 | 0.0318 |
| NM_008419 | potassium voltage-gated channel, member 5 (Kcna5) | Transport | −0.56587 | x 0.676 | 0.0363 |
| NM_007817 | cytochrome p450, 2f2 (Cyp2f2) | Enz, Detox | −0.56285 | x 0.677 | 0.0185 |
| NM_013494 | Carboxypeptidase E | Enz Prot metab | −0.56262 | x 0.677 | 0.0022 |
| NM_013712 | integrin beta 1 binding protein 2 | Recept, ECM | −0.56174 | x 0.677 | 0.0013 |
| NM_009814 | calsequestrin 2 (Casq2) | MCm | −0.55415 | x 0.681 | 0.0379 |
| BQ174827 | doublecortin and calciumcalmodulin-dependent protein kinase-like 1 | Enz, Prot metab | −0.55458 | x 0.68 | 0.0137 |
| NM_007429 | angiotensin II receptor, type 2 (Agtr2) | Recept | −0.55246 | x 0.682 | 0.0302 |
| NM_011098 | Brx1a mRNA for homeoprotein | Transcript, MCm | −0.54870 | x 0.684 | 0.0102 |
| AF356844 | carboxypeptidase Z | Enz, Prot metab | −0.54419 | x 0.685 | 0.0292 |
| NM_007751 | cytochrome c oxidase, subunit VIIIb (Cox8b) | Enz, Detox | −0.53763 | x 0.689 | 0.0133 |
| NM_011782 | a disintegrin-like and metalloprotease 5 (aggrecanase-2) (Adamts5) | Enz, ECM | −0.53751 | x 0.689 | 0.0316 |
| NM_023478 | uroplakin 3 (Upk3) | Epm | −0.53425 | x 0.69 | 0.0132 |
| NM_008664 | myomesin 2 | MCm | −0.53029 | x 0.692 | 0.0172 |
| NM_009610 | actin, gamma 2, smooth muscle, enteric (Actg2) | MCm | −0.52433 | x 0.695 | 0.0404 |
| NM_007868 | dystrophin, muscular dystrophy (Dmd) | MCm | −052267 | x 0.696 | 0.0067 |
| NM_080462. | histamine N-methyltransferase (Hnmt) | Enz, Immun | −0.52095 | x 0.697 | 0.0221 |
| NM_023617 | aldehyde oxidase 3 (Aox3) | Retino metab | −0.51687 | x 0.699 | 0.0094 |
| NM_025711 | asporin (Aspn) | ECM | −0.51470 | x 0.7 | 0.0146 |
| NM_007755 | cytoplasmic polyadenylation element binding protein (Cpeb) | Prot metab | −0.51347 | x 0.7 | 0.0004 |
| NM_017370 | haptoglobin (Hp) | Prot metab | −0.51060 | x 0.702 | 0.0175 |
| NM_008862 | protein kinase inhibitor, alpha (Pkia) | Prot metab | −0.50431 | x 0.705 | 0.0400 |
| NM_016762 | matrilin 2 | ECM | −050199 | x 0.706 | 0.0341 |
| NM_010390 | major histocompability complex Q1b | Immun | −0.50045 | x 0.707 | 0.0203 |
| NM_008242 | forkhead box D1 (Foxd1) | Transcript | −0.50023 | x 0.707 | 0.0268 |
| NM_008785 | serine (or cysteine) proteinase inhibitor, Clade A, member 5 (Serpina5) | Prot metab | −0.49974 | x 0.707 | 0.0366 |
| NM_009154 | semaphorin 5A (Sema5a) | Migr | −0.49740 | x 0.708 | 0.0409 |
| NM_020621 | pyrimidinergic receptor P2Y, G-protein coupled, 4 (P2ry4) | Recept | −049545 | x 0.709 | 0.0116 |
| M12233 | actin, alpha 1, skeletal muscle (Acta1) | MCm | −0.49534 | x 0.709 | 0.0005 |
| NM_008522.1 | lactotransferrin (Ltf) | Immun | −0.49078 | x 0,712 | 0.0160 |
| NM_053253.1 | Blu protein (Blu) | Immun | −0.48146 | x 0,716 | 0.0231 |
| NM_009144.1 | secreted frizzled-related sequence protein 2 (Sfrp2) | Prolif, EMT | −0.47671 | x 0,719 | 0.0211 |
| NM_011347.1 | selectin, platelet (Selp) | Recept, Adh | −0.47634 | x 0,719 | 0.0034 |
| NM_019662.1 | Ras-related associated with diabetes (Rrad) | Enz, GTPase | −0.47134 | x 0,721 | 0.0293 |
| NM_013669.1 | synaptosomal-associated protein, 91 kDa (Snap91) | Synapse marker | −0.46817 | x 0,723 | 0.0235 |
| NM_024406.1 | fatty acid binding protein 4, adipocyte (Fabp4) | Lipog | −0.46752 | x 0,723 | 0.0406 |
| AF357883.1 | homeodomain transcription factor (Nkx6-1) | Transcript | −0.46167 | x 0,726 | 0.0169 |
| AF020738.1 | fibroblast growth factor-related protein FGF-12A (Fgf12) | Sign | −0.45776 | x 0,728 | 0.0439 |
| NM_022881.1 | regulator of G-protein signaling 18 (Rgs18) | Signaling Regul | −0.45263 | x 0,731 | 0.0325 |
| NM_021467.2 | troponin I, skeletal, slow 1 (Tnni1) | MCm | −0.44982 | x 0,732 | 0.0163 |
| NM_019417.1 | PDZ and LIM domain 4 (Pdlim4) | Prot metab | −0.44649 | x 0,734 | 0.0225 |
| NM_010769.1 | matrilin 1, cartilage matrix protein 1 (Matn1) | ECM | −0.44476 | x 0,735 | 0.0280 |
| NM_019738.1 | nuclear protein 1 (Nupr1) | Transcript | −0.44316 | x 0,736 | 0.0051 |
| NM_023113.1 | aspartoacylase (aminoacylase) 2 | Enz, Prot metab | −0.43938 | x 0,737 | 0.0050 |
| NM_010669.1 | keratin complex 2, basic, gene 6b (Krt2-6b), | Epm | −0.43792 | x 0,738 | 0.0065 |
| NM_130895.1 | adenosine deaminase, RNA-specific, B1 (Adarb1) | Enz | −0.42746 | x 0,744 | 0.0152 |
| NM_031160.1 | ADP-ribosylation factor 4-like (Arf4l) | Signaling Regul | −0.42584 | x 0,744 | 0.0229 |
| NM_011582.1 | thrombospondin 4 (Thbs4) | ECM | −0.42420 | x 0,745 | 0.0242 |
| AF071068.1 | aromatic-L-amino-acid decarboxylase | Enz | −0.42030 | x 0,747 | 0.0194 |
| NM_010016.1 | decay accelerating factor 1 (Daf1) | Immun | −0.41955 | x 0,748 | 0.0062 |
| NM_011459.1 | serine protease inhibitor 8 (Spi8) | Enz | −0.41851 | x 0,748 | 0.0025 |
| NM_007940.1 | epoxide hydrolase 2, cytoplasmic (Ephx2) | Enz, Detox | −0.41824 | x 0,748 | 0.0242 |
| NM_007689.1 | chondroadherin (Chad) | ECM | −0.41822 | x 0,749 | 0.0327 |
Abbreviations: Funct. Cat., functional category of gene product; Adh, adhesion molecule; Detox, detoxification and xenobiotic metabolism; ECM, extracellular-matrix component or metabolism; Epm, epithelial marker; Enz, enzyme or enzyme inhibitor; Glycoconj, glycoconjugate metabolism; Immun, immune system; Inter metab, intermediary metabolism; Lipog, lipogenesis; MCm, muscle cell marker, Migr, cell migration; Prot metab, protein metabolism; Recept, receptor; Regul, regulator; Retino metab, retinoid metabolism, Signal, signaling molecule; Transcript, transcription factor or regulator; Transport, transporter molecule.
Genes with name and symbol in bold character had changes checked by RT-qPCR.