Figure 1.

Represents hybridization signals obtained from cloned-derived PCR biotinylated products from five Entamoeba species. (1) 5 μl of biotinylated amplicon diluted with 12 μl of TE buffer (pH 8) and (2) 17 μl of biotinylated amplicon diluted 1:100 in TE were used to determine the optimal conditions for hybridization and demonstrate that maximal MFI values were obtained with a hybridization temperature of 46°C, hybridization time of 60 min, SA-PE at a dilution of 1:50 preheated. Strains and probes tested were as follows: Microsphere coated with hist116 (E. histolytica), Dis 238 (E. dispar), Ecoli310 (E. coli), Ehart1 (E. hartmanni ) and Emoshk (E.moshovskii).