Table 1.
PCR primers and conditions
| Generic PCR | Primers (5′ -3′) | Cycling structure |
|---|---|---|
| JVF (forward) |
GTTGATCCTGCCAGTATTATATG |
95°C for 5 min followed by 40 cycles of 95°C for 30 s, 57°C for 30 s, 72 C for 1 min, 72 C for 7 min |
| DSPR2 B (reverse) |
CACTATTGGAGCTGGAATTAC |
|
| JVF (forward) |
GTTGATCCTGCCAGTATTATATG |
95°C for 5 min followed by 40 cycles of 95°C for 30 s, 50°C for 30 s, 72 C for 1 min, 72 C for 7 min |
| EntaRev 390* (reverse) |
ATTCCTCGTTATCCGTTAT |
|
| JVF (forward) |
GTTGATCCTGCCAGTATTATATG |
95°C for 5 min followed by 40 cycles of 95°C for 30 s, 55°C for 30 s, 72 C for 1 min, 72 C for 7 min |
| EntaRev417* (reverse) | AAAGCTCCTCTCCGATGT |
*tagged with biotin at the 3′.