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. 2013 Mar 6;3:6. doi: 10.1186/2044-5040-3-6

Table 1.

Quantification of Western blot analysis

  TSCmKO Ctrl Ratio Number of replicates
p-mTORS2448
 19 ± 2***
 10 ± 1
 1.9
 4
mTOR
 31 ± 6
 28 ± 5
 1.1
 4
P-4E-BP1S65
 23 ± 5***
 12 ± 3
 1.9
 4
4E-BP1
 34 ± 6
 39 ± 6
 0.9
 4
P-S6S235/S236
 53 ± 1***
 13 ± 6
 4
 4
S6
 48 ± 11
 42 ± 17
 1.1
 4
IRS-1
 6 ± 2***
 24 ± 1
 0.3
 4
P-PKB/ AktS473
 4 ± 2***
 22 ± 3
 0.2
 4
PKB/ Akt
 28 ± 7
 22 ± 6
 1.2
 4
FoxO1
 23 ± 5
 18 ± 3
 1.3
 4
FoxO3a
 16 ± 7
 17 ± 5
 0.9
 4
P-FoxO1T24/3aT23
 2 ± 7**/ 8 ± 3*
 11 ± 2/ 20 ± 5
 0.2/ 0.4
 4
P-PKB/ AktS473 (Den. TA)
 n.d.
 n.d.
 n.d.
 3
P-S6S235/S236 (Den. TA)
 47 ± 12***
 5 ± 2
 9.4
 3
P-PKB/ AktS473 (Den. Sol)
 n.d.
 n.d.
 n.d.
 3
P-S6S235/S236 (Den. Sol)
 41 ± 10**
 3 ± 5
 13.7
 3
 
 RAmKO
 Ctrl
 ratio
 number of replicates
P-PKB/ AktS473 (Den. TA)
 12 ± 2
 2 ± 1
 6
 3
P-S6S235/S236 (Den. TA)
 n.d.
 n.d.
 n.d.
 3
P-PKB/ AktS473 (Den. Sol)
 19 ± 3
 3 ± 2
 6.3
 3
P-S6S235/S236 (Den. Sol) n.d. n.d. n.d. 3

Proteins were extracted from soleus (Sol) and tibialis anterior (TA) muscles of 90-day-old TSCmKO, RAmKO or control (Ctrl) littermates. “Den.” denotes: denervated. “n.d” denotes: not detectable. The amount of total protein loaded onto the SDS-PAGE was adjusted and Western blots were additionally normalized to α-actinin levels. Numbers given represent average gray values ± SEM after subtraction of the background. “Ratio” represents the average gray value obtained from a knockout animal divided by the gray values from the control littermates. “Number of replicates” represents the number of knockout animals analyzed. The number of Ctrl littermates was always the same or higher than the values given. P-values were determined by Student’s t-test; * P <0.05, ** P <0.01, ***P <0.001.