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. 2013 Feb 8;42(4):1139–1150. doi: 10.3892/ijo.2013.1814

Figure 3.

Figure 3

RWJ67657 inhibits p38 signaling. (A) MCF-7TN-R cells were treated with increasing concentrations of RWJ67657 (0–10 μM) for 6 h and harvested for western blot analysis with anti-phospho-p38 or anti-phospho-ATF2 antibodies. (B) MCF-7TN-R cells were transfected with Gal4-CHOP and Gal4-luciferase for 6 h followed by treatment with increasing concentrations of RWJ67657 (0–10 μM). The following day cells were harvested for luciferase assay. Data points and error bars represent the mean ± SEM of three independent experiments in triplicate (**p<0.01). (C) MCF-7TN-R cells were transfected with NF-κB-luciferase for 6 h and pre-treated with increasing concentrations of RWJ67657 (0–10 μM) followed by treatment with or without TNF (1.0 ng/ml) overnight. The following day cells were harvested for luciferase activity. Data points and error bars represent the mean ± SEM of three independent experiments in triplicate.