Figure 4.

Inhibition of p38 blocks clonogenic survival and suppresses tumor growth. (A) MCF-7TN-R cells were plated for clonogenic survival assay, treated with increasing concentrations of RWJ67657 (0–10 μM) and allowed to grow until visible colonies appeared. Cells were then harvested, stained and counted for clonogenic survival. Data points and error bars represent the mean ± SEM of three independent experiments in triplicate (^**p<0.01, ^***p<0.001). (B) MCF-7TN-R cells (5×10⁵) were injected s.c. into immune-compromised mice. The mice treated with vehicle control or RWJ67657 (60 mg/kg) for 9 days. Tumor volume was monitored biweekly after palpable tumor formation (^*p<0.05).