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Western blot analysis of E-cadherin (120 kDa), Smad2/3 (55 kDa) and α-SMA (42 kDa). (A) α-SMA expression was higher in a-HPMCs than in HPMCs and was suppressed by pre-treatment with 5 nM PTX. (B and C) It was not clear whether the other antineoplastic agents affected α-SMA expression. Total expression of Smad2/3 was not affected by 48-h exposure to TGF-β1 in any of the experiments.
