Skip to main content
. 2013 Apr 10;8(4):e61056. doi: 10.1371/journal.pone.0061056

Figure 4. Overexpression of GPR87 up-regulates CD133 expression and enhances CSC-related properties.

Figure 4

(A) Representative examples of proliferation assays examining the effect of GPR87 overexpression in SMMC-7721 and HCC-LY5 cells. (B) The gross features of the tumor-bearing NOD/SCID mice orthotopically transplanted with 2×106 SMMC-7721-lenti-GPR87 and SMMC-7721-lenti- pWPXL cells after 6 weeks (n = 6 each group). (C) Transwell migration assay in SMMC-7721 and HCC-LY5 cells overexpressing GPR87. (D) Transwell matrigel invasion assay in SMMC-7721 and HCC-LY5 cells overexpressing GPR87. (E) Immunohistochemical staining of GPR87 and CD133 in HCC tissues with intrahepatic metastasis. (F) The table showed the correlation between CD133 and GPR87 expression in HCC tissues with intrahepatic metastasis.