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. Author manuscript; available in PMC: 2013 Apr 11.
Published in final edited form as: Nat Commun. 2011 Jul 12;2:390. doi: 10.1038/ncomms1381

Figure 1. Msgn1 lies downstream of Wnt3a/β-catenin signaling.

Figure 1

a) Hierarchical clustering was used to visualize differences in Msgn1 expression between E7.75-8 wildtype and Wnt3a−/− embryos (p ≤ 0.05) as detected by Affymetrix microarrays. Each column represents a pool of three stage-matched embryos. Upregulated (red) and downregulated (blue) gene expression is shown, with the magnitude of change reflected by the color intensity. Color scale bar represents log intensity values. (b, c) WISH analysis of Wnt3a expression in the PS ectoderm (b), and Msgn1 expression in the adjacent PS mesoderm (c), of E7.5 wildtype embryos. (d-g) WISH analysis of Msgn1 expression in the posterior PSM of Wnt3a+/− (d), Wnt3a−/− (e), T-Cre; Ctnnb1LOF (f), T-Cre; Ctnnb1GOF (g) embryos at E8.5. Note that Msgn1 expression in the posterior PSM defines the oscillatory field. (h) qPCR analysis of Msgn1 expression, normalized to Gapdh levels, in GFP-Bry ESC treated with recombinant Wnt3a in serum-free conditions. Error bars represent standard deviation of 3 samples. Abbr.: LOF, loss of function; GOF, gain of function. Scale bar = 100 μm.