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. Author manuscript; available in PMC: 2013 Apr 11.
Published in final edited form as: Nat Commun. 2011 Jul 12;2:390. doi: 10.1038/ncomms1381

Figure 5. Expression of Msgn1 in differentiating ESC rapidly induces Notch signaling genes.

Figure 5

(a) Microarray analysis of gene expression in F-Msgn1 EBs in the absence (blue) and presence of Dox (red) over a 48 hour timecourse. log2 ratios of the normalized expression levels of Dll1, Dll3, Notch1, Lfng, Nrarp and the housekeeping control Gapdh are presented. Lfng expression was variable in Dox treated and untreated cells, presumably reflecting the dynamic expression of a cyclic gene, but was generally elevated by F-Msgn1 expression. Error bars indicate standard deviation of 3 biological replicates. (b) qPCR analysis of Notch pathway gene expression in F-Msgn1 ESC and EBs validates the microarray results shown in (a). Here error bars indicate standard deviation of 3 replicates. Abbreviations: ES, embryonic stem cells; Dox, Doxycycline.