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. Author manuscript; available in PMC: 2013 Apr 11.
Published in final edited form as: Stem Cells. 2011 Oct;29(10):1580–1588. doi: 10.1002/stem.715

Figure 2. Modification of ICE mES cells with Myf5, and efficient myogenesis in vitro.

Figure 2

(A) Schematic overview of mouse myogenic differentiation protocol.

(B) Immunostaining for MyoD or sarcomeric myosin heavy chain (MHC) in progeny of iMyf5 mES cells or control parental A2Lox.cre mES cells at the final stage of differentiation (in 2% horse serum) in the presence or absence of doxycycline.

(C) Myogenic gene expression measured by qRTPCR in the same cells.

(D) Myogenic protein expression measured by Western Blotting in the same cells.