Figure 4. Coexpression of Myc-tagged GluR2 with eYFP–PICK1 causes BAR domain-dependent co-clustering.
Myc-tagged GluR2 was transiently expressed in COS7 cells alone (upper row), together with eYFP–PICK1 wt (middle row) or together with eYFP–PICK1 3KE (bottom row). Cells were fixed, permeabilized and stained with anti-myc for confocal microscopy. eYFP was visualized by YFP fluorescence. MycGluR2 is shown in red (left column) and eYFP–PICK1 in green (middle column). Right column shows the merged pictures. White bar = 10 μm. The data shown are representative of four experiments.