Fig. 4.
The genetic deletion of p47phox in mice normalises total superoxide and total ROS levels increased by oleate in islets. p47phox-null mice (KO) and their WT littermate controls were treated for 48 h with saline (SAL, 0.5 μl/min) or oleate (OLE, 0.4 μmol/min) to elevate plasma NEFA 1.5- to twofold. Total (a) and mitochondrial (b) superoxide levels (WT-saline [SAL], n = 7; WT-oleate [OLE], n = 6; KO-OLE, n = 6; KO-SAL, n = 4) and total ROS (c) levels (WT-SAL, n = 6; WT-OLE, n = 6; KO-OLE, n = 7; KO-SAL, n = 3) in freshly isolated islets of the mice treated as above. Data are expressed as mean % of SAL ± SEM; ‡‡ p < 0.01 WT-OLE vs all. The representative fluorescent images of islets imaged with HEt, MitoSOX and H2DCF-DA are also shown