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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cell Signal. 2013 Feb 14;25(5):1156–1165. doi: 10.1016/j.cellsig.2013.02.005

Figure 1. Knockout (KO) of the RKIP gene in mice.

Figure 1

(A) Schematic representation of the genomic structure of wild type RKIP and the trapped RKIP allele. The PCR primers for genotyping were indicated as a, b, and c. Primers a + b produce approximately 600 bp PCR fragments for detecting the RKIP gene trapped allele, and primers a + c produce 978 bp PCR fragments for detecting the RKIP wild-type allele. (B) PCR genotyping of offspring from a RKIP+/− intercross. The 978 bp product corresponds to the wild-type allele (Wt) and the 600 bp product corresponds to the mutant allele (Mut). (C) Western blot analysis with the anti-RKIP antibody of whole-cell protein lysates from E12.5 embryos derived from a RKIP+/− intercross.