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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cell Signal. 2013 Feb 14;25(5):1156–1165. doi: 10.1016/j.cellsig.2013.02.005

Figure 6. Depletion of RKIP rescues decreased MAPK signaling in B-Raf knockout primary keratinocytes.

Figure 6

Primary keratinocytes isolated form wild type (WT) and RKIP−/− and/or B-Raf−/− mice were serum-starved overnight prior to stimulation with EGF (5ng/ml) for different times as indicated. B-Raf, RKIP, pERK1/2 and GAPDH (loading control) in total cell lysates were detected by western blotting. The bar charts represent the densitometric quantification of pERK/GAPDH ratio in untreated (0) or EGF-treated keratinocytes from three independent experiments. Error bars represent S.D. *p<0.01.