Figure 3. IT administration of TLR ligands in C57Bl/6 mice produced robust TA.
TLR ligands were spinally delivered via IT injection at the L5 level in C57Bl/6 mice. The effect of intrathecal (IT) administration of TLR ligands (Supplemetary Figure 2) on tactile thresholds was measured by von Frey filament testing, using the up-down method (A–D). Data expressed as mean ± SEM (n=5–8 mice/group) and analyzed via 2-way ANOVA, followed by Bonferroni post hoc test to compare each time point to the IT saline group (*p<0.05; **p<0.01). The same IT saline group is represented in all four graphs.