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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Pain. 2013 Jan 30;154(5):733–742. doi: 10.1016/j.pain.2013.01.012

Figure 6. TLR activation in Tnf−/− mice.

Figure 6

TLR ligands were spinally delivered via IT injection at the L5 level in Tnf−/− mice. The effect of intrathecal (IT) administration of TLR ligands on tactile thresholds was measured by von Frey filament testing, using the up-down method. (A–D) Data expressed as mean ± SEM (n=5–8 mice/group) and analyzed via 2-way ANOVA, followed by Bonferroni post hoc test to compare each time point to the IT saline group (*p<0.05; **p<0.01). The same IT saline group is represented in all four graphs. (E) Hyperalgesic indices were calculated for each mouse using their individual baseline threshold and calculating the area under the curve. Data expressed as mean ± SEM. Hyperalgesic index was analyzed via 2-way ANOVA followed by Bonferroni post hoc test to compare each C57Bl/6 treatment group to the same Tnf−/− treatment group (*p<0.05; **p<0.01).