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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jan 11;58:126–133. doi: 10.1016/j.freeradbiomed.2012.12.020

Figure 5. p47phox does not migrate to the membrane in the presence of AANO2.

Figure 5

J774A.1 macrophages were incubated with AA (10 μM) or AANO2 (10 μM) as before. Cells were washed and activated with FITC-conjugated zymosan at 37°C. Then, permeated cells were incubated with rabbit anti-mouse p47phox antibody followed by the addition of a secondary Alexa Fluor 594 goat anti-mouse IgG antibody. The intracellular localization of the FITC-zymosan (green fluorescence) and p47phox (red fluorescence) was observed by confocal microscopy.