Table 1. Inhibition of NOX2 by AANO₂ in an in vivo model of inflammation.

Mice were treated with i/p thioglycolate and subcutaneous injected with AANO₂ and peritoneal macrophages were obtained at day 4 as explained in the experimental section. The formation of O₂^·− derived-H₂O₂ in PMA activated-macrophages was evaluated by Amplex Red as previously. DPI addition was performed to isolated macrophages 30 min before PMA addition. Data shown correspond to the mean ± SD, n=4 representative of at least 3 independent experiments.

Condition	NOX2 activity (%)
Vehicle treated mice	25.9 ± 12.8
AANO₂ treated mice	5.7 ± 3.9^a
Vehicle mice + PMA	100 ± 22.6
AANO₂ mice + PMA	21.7 ± 10.3^b
Vehicle mice + PMA + DPI	27.1 ± 6.4^b
AANO₂ mice + PMA + DPI	5.9 ± 1.9^b

indicates statistical difference compared to the Vehicle treated mice, p < 0.05.

indicates statistical difference compared to the Vehicle mice +PMA, p< 0.05.

Table 1. Inhibition of NOX2 by AANO2 in an in vivo model of inflammation.

Table 1. Inhibition of NOX2 by AANO₂ in an in vivo model of inflammation.