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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Traffic. 2013 Mar 12;14(5):599–609. doi: 10.1111/tra.12058

Figure 4. Expression of mutant forms of Rab14 influences the fusion of early and late endocytic vesicles in wild-type and LvsB-null cells.

Figure 4

Cells were given a pulse of FITC dextran followed by a 30 minute chase and then a second pulse with TRITC dextran. The fusion of endosomal vesicles was determined by colocalization of the two differently labeled dextrans as shown in Supplemental Figure 1. The fraction of vesicles containing both fluid phase markers were quantified in two independent experiments and shown as the mean +/− range. Expression of the active form of Rab14-(Q67L) caused a five-fold increase in the heterotypic fusion in wild-type cells but not in LvsB-null cells. Significantly, the expression of the inactive form of Rab14-(N121I) suppressed the heterotypic fusion shown in LvsB-null cells.