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. 2013 Apr 11;4:83. doi: 10.3389/fmicb.2013.00083

FIGURE 1.

FIGURE 1

(A) Diagram of BYV genome with gene functions shown. L-Pro, papain-like leader protease; MET, methyltransferase (capping enzyme); S1H, superfamily I helicase; RdRp, RNA-dependent RNA polymerase; p6, 6-kDa protein; Hsp70h, Hsp70 homolog; p64, 64-kDa protein; CPm, minor capsid protein; CP, capsid protein; p20, 20-kDa protein; p21, 21-kDa protein. Homologous CP, CPm, and the C-terminal domain of p64 are shown in the same color. (B) Proteolytic gene expression cassette including β-glucuronidase ORF (GUS) fused in frame to papain-like, self-cleaving domain of potivirus HC-Pro. (C) Recombinant gene expression cassette including native BYV promoter, green fluorescent protein ORF (GFP) and heterologous BYSV promoter. (D) Dual expression cassette including heterologous BYSV and GLRaV-2 promoters, ER-targeted GFP and red fluorescent protein containing nuclear localization signal. (E) Confocal laser scanning microscopic image of the N. benthamiana leaf cell infected with BYV vector that expresses GFPER and RFPNLS. Note the virus-induced, ER-derived, multivesicular body likely containing viral RNA replication complexes.