FIG 5 .

Chitin induced a tolerogenic phenotype on lung pDC in the absence of C3 and C3aR. Forty-eight hours following chitin exposure, lung homogenates were obtained and the expression of CD273, CD274, and CD86 on pDC was determined by flow cytometry. pDC were defined as CD11b⁻ CD11c^dim CD62L⁺ Siglec H⁺. (A) Expression of CD273 on lung pDC (n = 5). Values are mean fold change ± SEM of the mean fluorescent intensity (MFI) of chitin-exposed mice versus PBS-exposed mice. *, P < 0.05 KO versus wild type. (B) Expression of CD274 on lung pDC (n = 5). Values are mean fold change ± SEM of the MFI of chitin-exposed mice versus PBS-exposed mice. *, P < 0.05 for comparison of KO versus wild type. (C) The tolerogenic phenotype of pDC in the absence of C3 and C3aR. Values are the ratios ± SEM between the MFI of CD274 and the MFI of CD86 on lung pDC. *, P < 0.05 for comparison of chitin versus PBS.